Design, preparation, and selection of DNA-encoded dynamic libraries
نویسندگان
چکیده
منابع مشابه
Design, preparation, and selection of DNA-encoded dynamic libraries.
We report a method for the preparation and selection of DNA-encoded dynamic libraries (DEDLs). The library is composed of two sets of DNA-linked small molecules that are under dynamic exchange through DNA hybridization. Addition of the protein target shifted the equilibrium, favouring the assembly of high affinity bivalent binders. Notably, we introduced a novel locking mechanism to stop the dy...
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Biochemical combinatorial techniques such as phage display, RNA display and oligonucleotide aptamers have proven to be reliable methods for generation of ligands to protein targets. Adapting these techniques to small synthetic molecules has been a long-sought goal. We report the synthesis and interrogation of an 800-million-member DNA-encoded library in which small molecules are covalently atta...
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The discovery and development of novel drugs for the multitude of targets originating from functional genomic research is a challenging task. While antibodies can nowadays be raised against virtually any given target using phage-display methodologies, a similar "selection/amplification" approach for the facile discovery of low-molecular weight compounds capable of specific binding to protein ta...
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Driven by the need for new compounds to serve as biological probes and leads for therapeutic development and the growing accessibility of DNA technologies including high-throughput sequencing, many academic and industrial groups have begun to use DNA-encoded chemical libraries as a source of bioactive small molecules. In this review, we describe the technologies that have enabled the selection ...
متن کاملDesign, preparation, and selection of DNA-encoded dynamic libraries† †Electronic supplementary information (ESI) available: Materials and general methods, experimental details, library selection and sequencing methods and fold enrichment calculations. See DOI: 10.1039/c5sc02467f Click here for additional data file.
Key Laboratory of Bioorganic Chemistry and Education, Beijing National Laboratory of Chemistry and Molecular Engineering, Pe E-mail: [email protected] Key Laboratory of Chemical Genomics, Scho Peking University Shenzhen Graduate Schoo Biodynamic Optical Imaging Centre (BIOP University, Beijing, China 100871 † Electronic supplementary information methods, experimental details, library se fold ...
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ژورنال
عنوان ژورنال: Chemical Science
سال: 2015
ISSN: 2041-6520,2041-6539
DOI: 10.1039/c5sc02467f